Some features of the CaptureSelect hCG Affinity Matrix include:
Human gonadotropins (hCG, LH, FSH, TSH) are glycoprotein hormones secreted by gonadotrope cells of the pituitary gland that are often produced as recombinant proteins. They have an identical alpha chain subunit that is recognized by the ligand. Consequently, the CaptureSelect hCG Affinity Matrix shows binding to all human gonadotropins. The high selectivity and yields obtained using CaptureSelect hCG Affinity Matrix enable a robust and efficient purification process for hCG with excellent purity obtained in one step.
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect hCG affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: approx. 4 mg/mL resin (determined with HCG)
Elution conditions: 20 mM citric acid, pH 3.0
Caractéristiques principales
Selective for hCG, with cross-binding to LH, FSH, and TSH
Enables efficient purification of recombinant gonadotropins with high yields and purity
Offers excellent scalability
Non–animal-derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-158302
Réf. fabricant
30019983
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Conditions d’expédition
Room Temperature
Phase stationnaire
Anti-gonadotropin
Gamme de produits
CaptureSelect™
Type
Affinity Matrix
Quantité
50 mL
Forme
Agarose Bead
Granulométrie
65 ± 10 μm
Capacité de liaison
4 mg/mL
Débit
50 to 200 cm/hr. (Up to 2 bar)
Type de ligand
HCG affinity ligand
Matrice
Agarose-based, aldehyde-activated
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