Réf. : BL-RE-158305Réf. fabricant : 18871651 10 mL
1 596,00 € HT
CaptureSelect CH1-XL Affinity Matrix is the improved version of CaptureSelect IgG-CH1 Affinity Matrix. The specificity is the same, but it features a wider elution range (pH 3.0–4.5), increased binding capacity, and improved stability at higher pH.
Capacité de liaison
>15 mg/mL
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide-activated
Contenu et stockage
Store at 2°C to 8°C.
Conditions d’expédition
Approved for shipment at Room Temperature or on Wet Ice
Marque
Thermo Scientific
CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material.
CaptureSelect CH1-XL Affinity Matrix features include:
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect CH1-XL affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs lower capacities can be observed.
Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5
Caractéristiques principales
Platform approach for purification of IgGs and their Fab fragments directly from complex source materials in a single step with high purity and yield
Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
No co-purification of overproduced free light chain due to selectivity for the CH1 domain
No binding to bovine antibodies, so presence of fetal bovine serum is not a problem
Excellent scalability
Non-animal derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-158305
Réf. fabricant
18871651
Capacité de liaison
>15 mg/mL
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide-activated
Contenu et stockage
Store at 2°C to 8°C.
Conditions d’expédition
Approved for shipment at Room Temperature or on Wet Ice
Granulométrie
65 μm
Forme
Agarose Bead
Gamme de produits
CaptureSelect™
Quantité
10 mL
À utiliser avec (application)
IgG purification
Cible
CH1 domain of IgG
Type de ligand
CH1-XL affinity ligand
Phase stationnaire
Anti-IgG-CH1
Format
Bottle
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