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STEMCELL Technologies RoboSep™ Complete Kit for Human Whole Blood CD34+ Cells

Réf. : BL-RE-158362Réf. fabricant : 17167671 120 mL whole blood
1 789,50 € HT

Complete kit with RoboSep™ buffer and RoboSep™ filter tips

Type de cellule
Hematopoietic Stem and Progenitor Cells
À utiliser avec (application)
Cell Isolation
Quantité
120 mL whole blood
Marque
STEMCELL Technologies

Caractéristiques principales

  • The Complete Kit for Human Whole Blood CD34+ Cells is a simple two-step method that is designed to isolate CD34+ cells from whole blood or buffy coat
  • First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail (15186C) with antibodies recognizing CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD66b and CD61 surface markers
  • CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18066C), which contains an antibody recognizing CD34
  • RosetteSep™ binds unwanted cells to red blood cells (RBCs), forming immunorosettes, which sediment during density gradient centrifugation
  • The pre-enriched fraction containing CD34+ cells is harvested and then labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet
  • Isolated CD34+ cells are immediately available for downstream applications

Caractéristiques techniques

MarqueSTEMCELL Technologies
Réf. B-LABOBL-RE-158362
Réf. fabricant17167671
Type de celluleHematopoietic Stem and Progenitor Cells
À utiliser avec (application)Cell Isolation
Quantité120 mL whole blood
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