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Thermo Scientific™ 1-Step CHO High-Yield IVT Kit | B-LABO France
Thermo Scientific™ 1-Step CHO High-Yield IVT Kit
Réf. : BL-RE-158378Réf. fabricant : 15392607 2 x 100 μL
275,00 € HT
Express of functional proteins with much greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems using the Thermo Scientific™ 1-Step CHO High-Yield In Vitro Translation (IVT) Kit.
Description
1-Step™ CHO High-Yield IVT Kit, 2 x 100μL, Cell-Free Expression, Sufficient for 2 Reactions Of 100μL Each, –68 to –85°C Store In Ultra-Cold Freezer, Production of Large Amounts of Recombinant Protein for Structural Analysis
Formule
Multi-component kit
Contenus des kits
Lyophilized CHO lysate 1 vial, accessory proteins 25μL, dialysis buffer (4X) 900μL, reaction mix 40μL, positive control DNA (pCFE-GFP) 10μg, pT7CFE1-NHis-GST-CHA 10μg, microdialysis device 2 each, nuclease-free water 50mL
Rendement
750 Times greater yield per mL
Type
1-step CHO High-Yield IVT Kit
Marque
Thermo Scientific
The Thermo Scientific 1-Step CHO High-Yield In Vitro Translation (IVT) Kit enables the expression of functional proteins with up to 750 times greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems that only yield up to 10μg protein/mL reaction.
The 1-Step CHO High-Yield IVT System uses extracts containing the robust translation machinery of the pharmaceutical industry standard Chinese Hamster Ovary (CHO) cell line to generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids, and energy-generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750μg/mL. The CHO IVT kit includes all of the components required for transcription and translation of a recombinant gene, including an optimized expression vector.
Points forts
Includes
Lyophilized cell lysate, reaction mix, accessory proteins, 4X dialysis buffer, control DNA, cloning vector, dialysis devices, and centrifuge tubes.
Requires
30°C incubator or water bath (shaker incubator increases expression by 30 to 50%)
Applications
Production of large amounts of recombinant protein for structural analysis
Generation of active proteins for downstream applications such as protein interaction studies
Rapid large-scale expression and characterization of mutant proteins
Production of viral particles
Incorporation of unnatural amino acids
Production of cytotoxic proteins
Caractéristiques principales
High expression - up to 750μg/mL of expressed protein
Reproducible results - low variability between experiments
Fast - express high levels of protein with 6 hours to overnight incubation
Easier storage - allows you to store lyophilized CHO cell lysate at -20°C
Functional - obtain functionally active proteins, including those containing disulfide bonds
Easy-to-use - three reaction components together carry out transcription and translation in one step
Great for labeling - amenable to incorporation of labeled and unnatural amino acids
Vector compatibility - uses existing pT7CFE1 vectors for protein expression
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-158378
Réf. fabricant
15392607
Formule
Multi-component kit
Contenus des kits
Lyophilized CHO lysate 1 vial, accessory proteins 25μL, dialysis buffer (4X) 900μL, reaction mix 40μL, positive control DNA (pCFE-GFP) 10μg, pT7CFE1-NHis-GST-CHA 10μg, microdialysis device 2 each, nuclease-free water 50mL
Rendement
750 Times greater yield per mL
Type
1-step CHO High-Yield IVT Kit
Suffisant pour
2 Reactions of 100μL each
Quantité
2 x 100 μL
Contenu et stockage
Store in ultra-cold freezer (-68°C to -85°C)
À utiliser avec (application)
Protein biology, protein expression, cell-free (In-vitro) expression, In-vitro translation, production of large amounts of recombinant protein for structural analysis, generation of active proteins for downstream applications such as protein interaction s
