Defined medium for naïve-like human pluripotent stem cells
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500 mL Kit
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STEMCELL Technologies
RSeT™ Medium is a defined cell culture medium used for the reversion of primed human pluripotent stem cells (hPSCs) to a naïve-like state and maintenance of naïve-like hPSCs under feeder-dependent and hypoxic conditions
RSeT™ Medium contains pre-screened quality components and does not contain bFGF or TGFβ
It is compatible with human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells
hPSCs cultured in RSeT™ Medium exhibit features of a naïve-like state such as tightly packed, domed colonies with refractive edges
Key transcripts associated with naïve-like hPSCs such as KLF2, KLF4, and TFCP2L1 show increased expression in hPSCs cultured in RSeT™ Medium
RSeT™ hPSCs can be converted back to a primed state by culture in mTeSR™1 and can then be differentiated with STEMdiff™ products such as STEMdiff™ Definitive Endoderm Kit (Catalog #05110), STEMdiff™ SMADi Neural Induction Kit (Catalog #08581), and STEMdiff™ Mesoderm Induction Medium (Catalog #05220)
