The Ambion™ RiboPure™ Kit is designed for rapid purification of high-quality RNA from tissue samples or cultured cells. The kit combines TRI Reagent™ with glass fiber filter purification to yield exceptionally pure RNA, free of residual proteins and lipids. The kit includes sufficient reagents for 50 purifications from 1–100 mg of tissue or 0.1–20 x 106 cultured cells each.• Yields the highest purity RNA attainable• Ideal for the most stringent downstream applications• Handles problematic samples with ease• Fast (less than 30 minutes) and easy procedureRNA isolated using the RiboPure™ Kit is suitable for use in all common downstream applications, including cDNA synthesis, real-time and end-point RT-PCR, microarray analysis, northern blots, and RNase protection assays. The RiboPure™ protocol is especially suited for processing samples that are difficult to disrupt, or samples that have large amounts of ribonuclease or other contaminants. Samples are first homogenized in TRI Reagent™, a monophasic solution containing phenol and guanidine thiocyanate, to rapidly lyse cells and inactivate nucleases. Addition of bromochloropropane (BCP) results in separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, while DNA and protein remain in the interphase and organic phase. The aqueous phase is removed and passed through a glass fiber filter that binds the RNA. The filter is washed, and the RNA is eluted. The procedure is compatible with tissues that have been stored in Ambion's RNAlater™. Total RNA yield is typically 100–500 μg per 100 mg of tissue, depending on the type of tissue.
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Order Info
Conditions d’expédition : Température ambiante
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-159513 |
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| Réf. fabricant | 10321325 |
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| Contenu et stockage | • 50 mL TRI Reagent; store at 4°C<br />• 50 Filter Cartridges; room temperature<br />• 100 Collection Tubes (2 mL); room temperature<br />• 60 mL Wash Solution (concentrated); room temperature<br />• 11 mL Elution Buffer; room temperature<br />• Manual |
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| Isolation Technology | Spin Column (Glass Fiber Filter) |
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| Temps de purification | 30 min. (After sample homogenization) |
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| Volume d’élution | 100 μL |
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| Type d’échantillon | Cells, Tissue |
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| Type de produit final | Total RNA |
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| Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
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| À utiliser avec (application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
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| Comprend | TRI reagent, filter cartridges, 2 mL collection tubes, wash solution concentrate, elution buffer, and manual |
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| Type de produit | Kit with manual |
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| Conditions d’expédition | Room Temperature |
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| Quantité de matériel de démarrage | Cells: 0.1–20 x 10<sup>6</sup><br>Tissue: 5–100 mg |
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| Rendement | Up to 500 μg |
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