Building block for the lysine acylation using conjugating enzymes (LACE) technique
Ac-Cys-NHMe is a non-canonical amino acid that is an essential building block for lysine acylation using conjugating enzymes (LACE) technique. LACE is a chemoenzymatic approach that uses an E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme, Ubc9, to conjugate peptide thioesters containing a C-terminal ubiquitin-derived sequence (LRLRGG) to a lysine residue located in a recognition sequence (IKQE) on a protein via an isopeptide bond. Ac-Cys-NHMe can be reacted with a peptide hydrazide to form a reactive peptide thioester, which is the substrate for Ubc9. LACE enables the site-specific modification of proteins at lysine residues to allow attachment of biochemical probes such as fluorophores to proteins or to engineer proteins for site-specific delivery.
| Marque | Tocris Bioscience |
|---|---|
| Réf. B-LABO | BL-RE-160725 |
| Réf. fabricant | 30000025 |
| Nom chimique ou matériau | (2R)-2-(Acetylamino)-3-mercapto-N-methylpropanamide |
| CAS | 10061-65-1 |
| Quantité | 50 mg |
| Cible | Unnatural Amino Acids |
| Formule moléculaire | C<sub>6</sub>H<sub>12</sub>N<sub>2</sub>O<sub>2</sub>S |
