Bivalent SHAPE cross-linking reagent
TBIA (trans-bis-isatoic anhydride) is a bifunctional SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension) chemical cross-linking reagent. TBIA has two electrophilic isatoic anhydride moieties, both of which can react with the 2'-OH group in nucleotides adjacent to and distant from the primary RNA sequence. TBIA is used in SHAPE-JuMP (-juxtaposed merged pairs) a technique for exploring RNA tertiary structure proximity on large RNAs, and higher-order RNA-RNA interactions at nanometer resolution.
| Marque | Tocris Bioscience |
|---|---|
| Réf. B-LABO | BL-RE-160840 |
| Réf. fabricant | 30000435 |
| Nom chimique ou matériau | 1,6-Dihydrobenzo[1,2-d:4,5-d']bis([1,3]oxazine)-2,4,7,9-tetraone |
| Quantité | 10 mg |
| Cible | DNA, RNA and Protein Synthesis Compounds |
| Formule moléculaire | C<sub>10</sub>H<sub>4</sub>N<sub>2</sub>O<sub>6</sub> |
| Pureté | 95% |
