PreQ1 with biotin handle for RNA labeling and purification
preQ1-biotin is a modified analog of the natural substrate prequeosine1 (preQ1) and is used for RNA-TAG (transglycosylation at guanosine) and DNA-TAG. preQ1-biotin enables affinity tagging and pull-down of specific RNAs that have been modified selectively by
E. coli
tRNA guanine transglycosylase (TGT). preQ1-biotin is incorporated site-specifically and covalently into RNAs containing a short harpin nucleotide recognition motif. The guanine in a UGU recognition element is exchanged with the preQ1-biotin substrate.
Plasmids for expression of the E.coli TGT enzyme ( #138201 ) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end ( #138209 ) are available from Addgene.
| Marque | Tocris Bioscience |
|---|---|
| Réf. B-LABO | BL-RE-163095 |
| Réf. fabricant | 18758986 |
| Nom chimique ou matériau | N-(6-(((2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl)amino)hexyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide |
| Quantité | 5 mg |
| Cible | Buffers, Chelators and Reagents |
| Formule moléculaire | C<sub>23</sub>H<sub>36</sub>N<sub>8</sub>O<sub>3</sub>S |
