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Lonza™ P3 Primary Cell 96-well Nucleofector™ Kit

Réf. : BL-RE-166709Réf. fabricant : 11506539 2.25 mL P3 Primary Cell Nucleofector™ Solution · 0.5 mL Supplement 1…
1 412,73 € HT

For medium-throughput transfection of primary cells, e.g., human T cells, CD34 or rat neurons, when working with the 96-well Shuttle™ Add-On.

Couleur
Clear
Vector
pmaxGFP™
À utiliser avec (équipement)
96-well Shuttle™ Add-On
À utiliser avec (application)
Medium-throughput primary cell transfection
Comprend
P3 Primary Cell Nucleofector™ Solution, Supplement 1, pmaxGFP™ Vector (1μg/μL in 10mM Tris, pH 8.0; 50 μg), 96-well Nucleocuvette™ Plate
Marque
Lonza

Caractéristiques principales

  • High transfection efficiencies for primary cells.
  • Compatible with liquid handling systems for automated high-throughput electroporation.
  • Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System.
  • Applications:Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells.High-throughput applications, e.g., screens of arrayed cDNA, RNAi or CRISPR libraries.
  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells.
  • High-throughput applications, e.g., screens of arrayed cDNA, RNAi or CRISPR libraries.
  • This kit has been determined to be optimal for transfecting following cell types:B cell (human)CD34+ cell (human)Chondrocyte (human)DC (human)DC (mouse), matureES (human), H9ES (mouse)Hepatocyte (human)HMEC (human)Keratinocyte (human)Macrophage (human)Monocyte (human)Neuron (rat), brain Hi/CxNHBE (human)RPE (human)T cell (human), stim.T cell (mouse), unstim..
  • B cell (human)
  • CD34+ cell (human)
  • Chondrocyte (human)
  • DC (human)
  • DC (mouse), mature
  • ES (human), H9
  • ES (mouse)
  • Hepatocyte (human)
  • HMEC (human)

Caractéristiques techniques

MarqueLonza
Réf. B-LABOBL-RE-166709
Réf. fabricant11506539
CouleurClear
VectorpmaxGFP™
À utiliser avec (équipement)96-well Shuttle™ Add-On
À utiliser avec (application)Medium-throughput primary cell transfection
ComprendP3 Primary Cell Nucleofector™ Solution, Supplement 1, pmaxGFP™ Vector (1μg/μL in 10mM Tris, pH 8.0; 50 μg), 96-well Nucleocuvette™ Plate
Nbre de réactions96
pH7.1 to 7.3
Quantité2.25 mL P3 Primary Cell Nucleofector™ Solution, 0.5 mL Supplement 1, 50 μg pmax GFP™ Vector (1 μg/μL in 10 mM Tris pH 8.0), 1 x 96-well Nucleocuvette™ Plate (20 μL)
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