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Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Réf. : 30245027

76.70€ - 1070.00€€

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Type de produit: Reaction Buffer
Forme: Liquid
À utiliser avec (application): RPA, RT-RPA
Quantité: 2000 μL
Catégorie de recherche: Nucleic Acid Amplification
Marque: Invitrogen™

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
Robust and specific RNA/DNA amplification
Amplification from inhibitor-containing samples
Fast target amplification in as little as 20 minutes
Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
Optimized reaction buffer, magnesium chloride, and dNTPs

Type de produit: Reaction Buffer
Forme: Liquid
À utiliser avec (application): RPA, RT-RPA
Quantité: 2000 μL
Catégorie de recherche: Nucleic Acid Amplification
Contenu et stockage: 2X RPA Reaction Buffer, 2 mL, Store at -25°C to -15°C.
Température de réaction optimale: 42°C
Nom du produit: RPA Reaction Buffer (2X)
Conditions d’expédition: Dry Ice
Concentration: 2X
Temps de réaction: Up to 20 min.

Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

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