For the quantitative detection of IL-1 beta Mouse
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-171993 |
| Réf. fabricant | 18031673 |
| Numéro d’adhésion | P10749 |
| Plage de dosage | 7.8 to 500 |
| Sensibilité du dosage | 1.2 pg/mL |
| Conjugué | HRP |
| Type de produit | ELISA |
| Type d’échantillon | Serum |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 16176 |
| Symbole de gène(s) | IL1B |
| Coefficient de variation inter-dosage | 5.7% |
| Coefficient de variation intra-dosage | 5.1% |
| Contenus des kits | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Volume d’échantillon | 50 µL |
| Conditions de stockage | 2°C to 8°C |
| Cible | Mouse |
| Heure du test | 1 hr 20 min |
| Durée totale du dosage | 3 hr 10 min |
