For the quantitative detection of Human IL-17A
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-171995 |
| Réf. fabricant | 18031683 |
| Numéro d’adhésion | Q16552 |
| Plage de dosage | 0.23 to 15 pg/ml |
| Sensibilité du dosage | 0.01 pg/mL |
| Conjugué | HRP |
| Type de produit | ELISA |
| Type d’échantillon | Supernatant |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 3605 |
| Symbole de gène(s) | IL17A |
| Coefficient de variation inter-dosage | 6.3% |
| Coefficient de variation intra-dosage | 8.5% |
| Contenus des kits | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Amplification Reagent I, Amplification Reagent II, SAV-HRP, Wash Buffer, Chromogen Stop Solution Adhesive Plate Covers |
| Marqueur ou colorant | HRP |
| Quantité | 96 Tests |
| État réglementaire | RUO |
| Volume d’échantillon | 50 µL |
| Conditions de stockage | 2°C to 8°C |
| Cible | Human |
| Heure du test | 1 hr 20 min |
| Durée totale du dosage | 4 hr |
