Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
Numéro d’adhésion : Q16552
Plage de dosage : 0.23 to 15 pg/ml
Sensibilité du dosage : 0.01 pg/mL
Conjugué : HRP
Type de produit : ELISA
Type d’échantillon : Supernatant
À utiliser avec (équipement) : Colorimetric Microplate Reader
Identification génétique (Entrez) : 3605
Symbole de gène(s) : IL17A
Coefficient de variation inter-dosage : 6.3%
Coefficient de variation intra-dosage : 8.5%
Contenus des kits : Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Amplification Reagent I, Amplification Reagent II, SAV-HRP, Wash Buffer, Chromogen Stop Solution Adhesive Plate Covers
Marqueur ou colorant : HRP
Quantité : 96 Tests
État réglementaire : RUO
Volume d’échantillon : 50 µL
Conditions de stockage : 2°C to 8°C
Cible : Human
Heure du test : 1 hr 20 min
Durée totale du dosage : 4 hr
