Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Proteins from any expression system can be purified under native or denaturing conditions.
Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
The resin is precharged with Ni2+ ions and therefore ready to use.
Technology: Immobilized metal ion affinity chromatography
