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Thermo Scientific™ Solution d’arrêt de dosage de l… | B-LABO France
Thermo Scientific™ Solution d’arrêt de dosage de la bêta-galactosidase
Réf. : BL-RE-174757Réf. fabricant : 11517830 25 ml
81,25 € HT
The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.
Description
Solution d’arrêt de dosage bêta-galactosidase, 25 ml, pH de 11,05, liquide, transparent, plaque à 96 puits, type de dosage enzymatique rapporteur, méthode de détection colorimétrique, cellules compatibles avec les mammifères, substrat exclusif, substrat Beta-Gal, technique d’absorbance
Contenu et stockage
Conserver à température ambiante.
Formule
Liquide
À utiliser avec (application)
Biologie des protéines, dosages de rapporteurs d’enzymes, dosages et analyses des protéines, dosages de bêta-galactosidase, Absorbance
Méthode de détection
Enzyme rapporteur
Marque
Thermo Scientific
The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):
