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The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.
The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.
The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):
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Description : Solution d’arrêt de dosage bêta-galactosidase, 25 ml, pH de 11,05, liquide, transparent, plaque à 96 puits, type de dosage enzymatique rapporteur, méthode de détection colorimétrique, cellules compatibles avec les mammifères, substrat exclusif, substrat Beta-Gal, technique d’absorbance
Contenu et stockage : Conserver à température ambiante.
Formule : Liquide
À utiliser avec (application) : Biologie des protéines, dosages de rapporteurs d’enzymes, dosages et analyses des protéines, dosages de bêta-galactosidase, Absorbance
Méthode de détection : Enzyme rapporteur
Plage de pH : 11,05
Volume d’échantillon : 25 ml
À utiliser avec (équipement) : Microplate Reader
Type de produit : Solution d’arrêt de dosage de la bêta-galactosidase
Type de colorant : Marquage enzymatique
Format : Plaque 96 puits
Quantité : 25 ml
Gamme de produits : Pierce™
Substrat : Exclusif
Type de substrat : Substrat Bêta-Gal
Cible : La bêta-galactosidase
