The Applied Biosystems™ MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 dried blood spots is designed to recover viral RNA from dried blood spots (DBS) and plasma for HIV-1 drug resistance testing.
Type de microbilles
Magnetic
Contenu et stockage
• 60 mL Dried Blood Spots Lysis Solution • 55 mL Binding Solution • 100 mL Wash Buffer • 10 mL Elution Solution • 1 mL Proteinase K • 2 mL Total Nucleic Acid Binding Beads
Méthode de détection
HIV-1 viral RNA compatible with downstream applications such as RT-PCR and Sanger sequencing
Type d’échantillon
Whole Blood, Plasma
À utiliser avec (équipement)
KingFisher Duo Prime, KingFisher Flex, Magnetic Stand
Marque
Applied Biosystems
Points forts
Benefits of MagMAX magnetic bead–based purification
Beads bind the nucleic acid more efficiently than glass-fiber filters, resulting in higher and more consistent yields
Since filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples
This clogging issue is of particular concern with protein-rich, large-volume samples such as whole blood or plasma that are commonly used for viral testing
Caractéristiques principales
Kit is designed to recover viral RNA from dried blood spots (DBS) and plasma for HIV-1 drug resistance testing
MagMAX magnetic-bead technology ensures reproducible recovery of high-quality HIV-1 viral RNA compatible with downstream applications such as RT-PCR and Sanger sequencing
Automation: Workflows are designed for the KingFisher Flex and KingFisher Duo Prime systems
Sensitivity: Extracts amplifiable viral RNA from DBS and plasma samplesDBS: >2000 copies/mLPlasma: >1000 copies/mL
DBS: >2000 copies/mL
Plasma: >1000 copies/mL
Duration: Test takes less than 60 minutes: ∼30 min DBS lysis, ∼30 min viral RNA extraction
Caractéristiques techniques
Marque
Applied Biosystems
Réf. B-LABO
BL-RE-174895
Réf. fabricant
17856501
Type de microbilles
Magnetic
Contenu et stockage
• 60 mL Dried Blood Spots Lysis Solution<br> • 55 mL Binding Solution<br> • 100 mL Wash Buffer<br> • 10 mL Elution Solution<br> • 1 mL Proteinase K<br> • 2 mL Total Nucleic Acid Binding Beads<br><br>
Méthode de détection
HIV-1 viral RNA compatible with downstream applications such as RT-PCR and Sanger sequencing
Type d’échantillon
Whole Blood, Plasma
À utiliser avec (équipement)
KingFisher Duo Prime, KingFisher Flex, Magnetic Stand
Nbre de réactions
100 reactions
Quantité
100 reactions
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