Invitrogen ProcartaPlex Human MEK1[pS218/pS222] Simplex Kit enables precise analysis of the Protein of Interest.
The ProcartaPlex Human MEK1[pS218/pS222] Simplex Kit measures the level of phosphorylated MEK1[pS218/pS222] in cell lysates. It is designed to be combinable with other Simplex kits so you can create your own multiplex panel that utilizes Luminex xMAP technology for protein quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.
For preparation of cell lysates, we recommend the use of either ProcartaPlex Cell Lysis Buffer (Cat. No. EPX-99999-000) or Cell Extraction Buffer (Cat. No. FNN0011) supplemented with 0.5 M EDTA and Halt Protease and Phosphatase Inhibitor Single to Use Cocktail 100X (Cat. No. 78440 or 78442). These buffers are not included in the kit. Depending on your cell system, other buffers can be suitable as well.
The ProcartaPlex Human MEK1[pS218/pS222] Simplex Kit is part of the ProcartaPlex Human MAPK Pathway Panel (phospho) 6plex (Cat. No. EPX060-97117-PHO), which complements the ProcartaPlex Human MAPK Pathway Panel (total) 6plex (Cat. No. EPX060-97017-901).
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-175239 |
| Réf. fabricant | 30275912 |
| Type de produit | Kit simplex |
| Quantité | 96 dosages |
| Espèces | Human |
| Combinability | Combinable |
| Type de microbilles | Magnetic |
| Zone des microbilles | MEK1[pS218/pS222] [63] |
| Sensibilité du dosage | Voir le certificat d’analyse |
| Plage de dosage | Voir le certificat d’analyse |
| À utiliser avec (équipement) | Instruments Luminex™ |
| Expression | MAP2K1 |
| Alias de gène | 5604 |
| Identification génétique (Entrez) | MAP2K1 |
| Compatibilité à haut débit | Yes |
| Coefficient de variation inter-dosage | See Certificate of Analysis |
| Coefficient de variation intra-dosage | See Certificate of Analysis |
| Gamme de produits | ProcartaPlex™ |
| Type d’échantillon | Cell lysates |
| Volume d’échantillon | Cell lysates (prediluted): 25 μL |
| ID UniProt | Q02750 |
| Synonyme | Mitogen-activated protein kinase |
| Contenu et stockage | 1 vial Capture Beads (50x)<br>1 vial biotinylated Detection Antibody (50x)<br>2 vials Standard Mix (lyophilized)<br><br>Store as indicated on the Certificate of Analysis. |
| Conditions d’expédition | Wet Ice |
| Research Area | Signaling |
