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Invitrogen ProcartaPlex Human JNK1/3 Simplex kit enables precise analysis of the Protein of Interest.
The ProcartaPlex Human JNK1/3 Simplex Kit measures the level of total JNK1/3 in cell lysates. It is designed to be combinable with other Simplex kits so you can create your own multiplex panel that utilizes Luminex xMAP technology for protein quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.
For preparation of cell lysates, we recommend the use of either ProcartaPlex Cell Lysis Buffer (Cat. No. EPX-99999-000) or Cell Extraction Buffer (Cat. No. FNN0011) supplemented with 0.5 M EDTA and Halt Protease and Phosphatase Inhibitor Single to Use Cocktail 100X (Cat. No. 78440 or 78442). These buffers are not included with the kit. Depending on your cell system, other buffers can be suitable as well.
The ProcartaPlex Human JNK1/3 Simplex Kit is part of the ProcartaPlex Human MAPK Pathway Panel (total) 6plex (Cat. No. EPX060-97017-901), which complements the ProcartaPlex Human MAPK Pathway Panel (phospho) 6plex (Cat. No. EPX060-97117-PHO).
About ProcartaPlex Assays for the Luminex Platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.
ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25 to 50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.
The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin to R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.
The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.
Type de produit : Kit simplex
Quantité : 96 dosages
Espèces : Human
Combinability : Non combinable
Type de microbilles : Magnetic
Zone des microbilles : JNK1/3 [13]
Sensibilité du dosage : Voir le certificat d’analyse
Plage de dosage : Voir le certificat d’analyse
À utiliser avec (équipement) : Instruments Luminex™
Expression : MAPK8
Alias de gène : JNK1, PRKM8
Identification génétique (Entrez) : 5599
Symbole de gène(s) : MAPK8
Compatibilité à haut débit : Yes
Coefficient de variation inter-dosage : See Certificate of Analysis
Coefficient de variation intra-dosage : See Certificate of Analysis
Gamme de produits : ProcartaPlex™
Type d’échantillon : Cell lysates
Volume d’échantillon : Cell lysates (prediluted): 25 μL
ID UniProt : P45983
Synonyme : SAPK, c-Jun-N-terminale Kinase, stress-activated phospho-kinase
Contenu et stockage : 1 vial Capture Beads (50x)
1 vial biotinylated Detection Antibody (50x)
2 vials Standard Mix (lyophilized)
Store as indicated on the Certificate of Analysis.
Conditions d’expédition : Wet Ice
Research Area : Signaling
