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Thermo Scientific™ CaptureSelect™ LambdaXP MiniChrom™ Pre-packed Column

Réf. : BL-RE-175258Réf. fabricant : 30276685 1 mL
495,00 € HT

Thermo Scientific™ CaptureSelect™ LambdaXP MiniChrom™ columns are designed for bench-scale resin screening, process development, and sample preparation.

Volume du lit
1 mL
Capacité de liaison
>30 g IgG/L
Débit
50 to 150 cm/hr.
Matrice
Agarose-based, Epoxide-activated
Diamètre (métrique)
0.8 cm
Marque
Thermo Scientific

CaptureSelect LambdaXP MiniChrom™ columns are designed for bench-scale resin screening, process development, and sample preparation.

These pre-packed columns are ready to connect and have the following features:

The 1-mL columns can be used for quick screenings of application feasibility and lab-scale purification on a convenient and easy-to-use pre-packed column. The 10-cm bed height of the 5-mL columns allows initial process development on a bench scale.

CaptureSelect LambdaXP affinity matrix recognizes the constant domain of the human lambda light chain enabling purification of all IgG isotypes including IgG, IgA, IgM, IgD, and IgE directly from complex source materials in a robust and efficient purification process with excellent purity obtained in a single step.

Points forts

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect LambdaXP affinity ligand
  • Ligand coupling: epoxide coupling
  • Binding capacity: >30 g IgG/L resin depending on flow rate, column height, and contact time
  • Elution conditions: 25 mM sodium acetate, pH 3.5

Caractéristiques principales

  • Dimensions of 1-mL column: 0.8 x 2 cm
  • Dimensions of 5-mL column: 0.8 x 10 cm
  • Direct connection to standard chromatography systems
  • Packed with CaptureSelect LambdaXP Affinity Matrix, offering high dynamic binding capacity
  • Efficient elution of Fab fragments and immunoglobulins containing a lambda light chain, no cross binding to the Fc-region or kappa light chains
  • One-step purification from crude material with high purity and yield
  • Human specific
  • Non–animal-derived

Caractéristiques techniques

MarqueThermo Scientific
Réf. B-LABOBL-RE-175258
Réf. fabricant30276685
Volume du lit1 mL
Capacité de liaison>30 g IgG/L
Débit50 to 150 cm/hr.
MatriceAgarose-based, Epoxide-activated
Diamètre (métrique)0.8 cm
Pression de fonctionnement max.2 bar
Longueur (métrique)2 cm
Granulométrie65 ±10 μm
Quantité1 mL
À utiliser avec (application)Chromatography
Type de ligandCaptureSelect™ LambdaXP Affinity
Phase stationnaireAgarose bead
FormatColumn
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