Thermo Scientific™ CaptureSelect™ LambdaXP Ligand Leakage ELISA detects possible presence of LambdaXP affinity ligand in the purified product after using CaptureSelect LambdaXP Affinity Matrix.
CaptureSelect LambdaXP Ligand Leakage ELISA is designed to detect possible leached ligand of human Fab fragments or Ig's with a lambda light chain that have been purified using CaptureSelect LambdaXP Affinity Matrix.
The LambdaXP affinity ligand is a 13-kD recombinant protein, expressed in yeast and covalently coupled to a chromatography matrix to give high chemical stability and low leakage. During the chromatography process small amounts of the affinity ligand can leach from the chromatography support and co-elute with the protein. This ELISA provides typical sensitivity and accurate quantitation of possible leached ligand greater than or equal to 1 ng/mL. The CaptureSelect LambdaXP Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
