Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.
Volume du lit
20 mL
Capacité (métrique)
34 mL
Matériau
Polypropylène
Contenu et stockage
Température ambiante
Gamme de produits
Pierce™
Marque
Thermo Scientific
Points forts
Pierce Centrifuge Columns
Thermo Scientific Pierce centrifuge columns are convenient tools for handling 40 μL to 20 mL of an affinity support for purification. The columns are made from low-protein-binding polypropylene and contain a polyethylene filter (~30-μm pore size) for compatibility with protein purification.
Simply add the affinity resin to the polypropylene column, remove the twist-off bottom, and allow the resin to pack itself. Then add your sample, allow it to bind to the support, and use a centrifuge to efficiently wash away any contaminants and elute your purified protein.
0.8 mL columns
Maximum volume: 800 μL
Recommended resin volume: 40–400 μL
Receiver tube: fits standard microcentrifuge tubes
Cap: O-ring screw-top
Twist-off bottom
2 mL columns
Maximum volume: 5 mL (2 mL resin bed, 3 mL sample)
Recommended resin volume: 400 μL–2 mL
Receiver tube: fits standard 15 mL conical centrifuge tubes
Cap: screw-top
Twist-off bottom and press-on cap to reseal
5 mL columns
Maximum volume: 8 mL (5 mL resin bed, 3 mL sample)
Recommended resin volume: 1–5 mL
Receiver tube: fits standard 15 mL conical centrifuge tubes
Cap: screw-top
Twist-off bottom and press-on cap to reseal
10 mL columns
Maximum volume: 22 mL (10 mL resin bed, 12 mL sample)
Recommended resin volume: 2–10 mL
Receiver tube: fits standard 50 mL conical centrifuge tubes
Cap: screw-top
Twist-off bottom and press-on cap to reseal
20 mL columns
Maximum volume: 34 mL (20 mL resin bed, 14 mL sample)
Recommended resin volume: 4–20 mL
Receiver tube: fits standard 50 mL conical centrifuge tubes
Cap: screw-top
Twist-off bottom and press-on cap to reseal
Applications
Affinity purification, affinity chromatography
Immuno-depletion
Ion exchange resins
Gel filtration, desalting, buffer exchange
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-175271
Réf. fabricant
30276438
Volume du lit
20 mL
Capacité (métrique)
34 mL
Matériau
Polypropylène
Contenu et stockage
Température ambiante
Gamme de produits
Pierce™
Quantité
25 colonnes
Cible
Batch Binding
Type
Spin Column
Volume d’échantillon
14 mL
Format
Spin Column
Type de colonne
Centrifuge Column
Une question, une configuration particulière ? Contactez-nous pour un devis.
