ELISA
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-177735 |
| Réf. fabricant | 30284441 |
| Numéro d’adhésion | P07196, P08551, P19527 |
| Plage de dosage | 0.48 to 7500 pg/mL |
| Sensibilité du dosage | 0.48 pg/mL |
| Conjugué | FAM™ |
| Type de produit | ProQuantum |
| Type d’échantillon | Plasma, Serum, Supernatant |
| À utiliser avec (équipement) | qPCR Instruments |
| Identification génétique (Entrez) | 18039, 4747, 83613 |
| Symbole de gène(s) | NEFL |
| Contenus des kits | Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase |
| Quantité | 5 X 96 Tests |
| État réglementaire | RUO |
| Volume d’échantillon | Plasma: 2 to 5 μL, Serum: 2 to 5 μL, Supernatant: 2 to 5 μL |
| Conditions de stockage | -20°C |
| Cible | NEFL |
| Heure du test | 30 min. |
| Durée totale du dosage | 2 hr. |
