ELISA
The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-177850 |
| Réf. fabricant | 30245263 |
| Plage de dosage | 0.16 to 10 ng/mL |
| Sensibilité du dosage | 0.09 ng/mL |
| Conjugué | HRP |
| Type de produit | ELISA |
| Type d’échantillon | Plasma, Serum |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Coefficient de variation inter-dosage | <10 |
| Coefficient de variation intra-dosage | <10 |
| Contenus des kits | Micro ELISA Plate (Dismountable), Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis |
| Marqueur ou colorant | Biotin |
| Quantité | 96 Tests |
| État réglementaire | RUO |
| Volume d’échantillon | Plasma: 100 μL, Serum: 100 μL |
| Conditions de stockage | Refer to product documentation for component specific storage temperature. |
| Cible | Complement C5a |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 3 hr. 30 min. |
