ELISA
The complement system is a fundamental part of innate immunity, consisting of over 45 genes that encode various complement components, regulators, and receptors. The complement C5b-9 complex, also known as the Membrane Attack Complex (MAC), plays a crucial role in the immune defense mechanism. It includes the sequential assembly of proteins C5b, C6, C7, C8, and multiple copies of C9. This complex forms transmembrane channels that puncture the cell membrane of target cells, leading to cell lysis. Specifically, MAC has significant implications in mediating cell death in both pathogenic organisms and cancer cells. The genes involved are clustered across the human genome, mainly organized in three significant loci. The understanding of the C5b-9 complex's function extends beyond its antimicrobial roles to include its interactions with tumor cells, where it can induce cell death and influence cancer progression.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-177856 |
| Réf. fabricant | 30245133 |
| Numéro d’adhésion | P06683 |
| Plage de dosage | 0.31 to 20 ng/mL |
| Sensibilité du dosage | 0.19 ng/mL |
| Conjugué | HRP |
| Type de produit | ELISA |
| Type d’échantillon | Plasma, Serum |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 109828, 12274, 12279 |
| Symbole de gène(s) | C6, C7, C9 |
| Coefficient de variation inter-dosage | <10 |
| Coefficient de variation intra-dosage | <10 |
| Contenus des kits | Micro ELISA Plate (Dismountable), Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis |
| Marqueur ou colorant | Biotin |
| Quantité | 96 Tests |
| État réglementaire | RUO |
| Volume d’échantillon | Plasma: 100 μL, Serum: 100 μL |
| Conditions de stockage | Refer to product documentation for component specific storage temperature. |
| Cible | Complement C5b-9 |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 3 hr. 30 min. |
