ELISA
Heparan sulfate (HS) is a glycosaminoglycan that is ubiquitously expressed on cell surfaces and in the extracellular matrix and basement membrane. Each HS molecule is a linear polysaccharide composed of repeating disaccharides of hexuronic acid and d-glucosamine that can exhibit immense structural diversity due to substitution to varying extents with sulfate groups and epimerization of glucuronic acid to iduronic acid, with areas of high sulfation and glucuronic acid epimerization being co-located in hot spots throughout the molecule. HS is structurally related to heparin, an extremely highly sulfated form of HS that is restricted to mast cells. The biosynthesis and modification of HS chains is thought to take place within the endoplasmic reticulum, Golgi apparatus, and trans Golgi network, which in the end produce unique HS chains that are covalently attached to a range of core proteins to form HS-proteoglycans [PMID: 24391644].
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-177866 |
| Réf. fabricant | 30245000 |
| Plage de dosage | 0.31 to 20 ng/mL |
| Sensibilité du dosage | 0.19 ng/mL |
| Conjugué | HRP |
| Type de produit | ELISA |
| Type d’échantillon | Plasma, Serum |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Coefficient de variation inter-dosage | <10 |
| Coefficient de variation intra-dosage | <10 |
| Contenus des kits | Micro ELISA Plate (Dismountable), Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis |
| Marqueur ou colorant | Biotin |
| Quantité | 96 Tests |
| État réglementaire | RUO |
| Volume d’échantillon | Plasma: 100 μL, Serum: 100 μL |
| Conditions de stockage | Refer to product documentation for component specific storage temperature. |
| Cible | N-sulfated Heparan Sulfate |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 3 hr. 30 min. |
