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R&D Systems™ Cultrex RGF Basement Membrane Extract, Type 2

Réf. : BL-RE-031658Réf. fabricant : 18776937 5 mL
294,81 € HT

For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract

Contenu et stockage
Product is stable for a minimum of 3 months from date of shipment when stored at -20°C in a manual defrost freezer. For optimal stability, store at -80°C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 μg/mL gentamicin sulfate. Sterility Testing: PathClear™ - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation (at 37°C for 14 days following USP sterility testing guidelines.)
Concentration
12 to 18 mg/mL
À utiliser avec (application)
Bioactivity
Quantité
5 mL
Source
Murine Engelbreth-Holm-Swarm (EHS) tumor.
Marque
R&D Systems

Points forts

Functional Assay -

Tube formation assay: BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.

Gelling Assay

- BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.

Caractéristiques techniques

MarqueR&D Systems
Réf. B-LABOBL-RE-031658
Réf. fabricant18776937
Contenu et stockageProduct is stable for a minimum of 3 months from date of shipment when stored at -20°C in a manual defrost freezer. For optimal stability, store at -80°C. Avoid freeze-thaw cycles. <b>Storage Buffer:</b> Dulbecco’s Modified Eagle’s medium without phenol red, with 10 μg/mL gentamicin sulfate. <b>Sterility Testing:</b> PathClear™ - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation (at 37°C for 14 days following USP sterility testing guidelines.)
Concentration12 to 18 mg/mL
À utiliser avec (application)Bioactivity
Quantité5 mL
SourceMurine Engelbreth-Holm-Swarm (EHS) tumor.
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