Generate self-organized, pluripotent stem cell (PSC)-derived neural organoids with a cellular composition and structural organization representative of the developing human brain
This simple, four-stage protocol is based on the formulation published by Lancaster et al. (Lancaster MA et al. Nature, 2013 and Lancaster MA et al. Science, 2014) to more reliably generate cerebral organoids
Beginning with an embryoid body (EB) formation step followed by expansion of neuroepithelia, organoids generated using STEMdiff Cerebral Organoid Kit feature cortical-like regions including the ventricular zone, outer subventricular zone, intermediate zone, and cortical plate
For extended culture periods (> 40 days), the components required for maturation can be purchased as STEMdiff Cerebral Organoid Maturation Kit (Catalog #08571)
