For differentiation of human PSCs into the hematopoietic lineage
Type de cellule
Hematopoietic Stem and Progenitor Cells, Pluripotent Stem Cells
À utiliser avec (application)
Cell Culture, Differentiation
Quantité
1 Kit
Marque
STEMCELL Technologies
Caractéristiques principales
Generate functional hematopoietic progenitor cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells with the STEMdiff Hematopoietic Kit
Using a simple and reproducible protocol in serum- and feeder-free conditions, you can produce hematopoietic progenitor cells expressing CD34, CD45, and CD43
The robust, two-stage protocol initially induces cells toward the mesoderm before further differentiating them into hematopoietic progenitor cells
After 12 days, a population of hematopoietic cells containing 25 - 65% (average 43%) CD34+CD45+ progenitor cells can be harvested, including progenitor cells that have the capacity to form functional hematopoietic colonies in the colony-forming unit (CFU) assay
The STEMdiff Hematopoietic Kit has been optimized for differentiation of human pluripotent cells maintained in mTeSR1 (Catalog #85850)
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-031749
Réf. fabricant
16699585
Type de cellule
Hematopoietic Stem and Progenitor Cells, Pluripotent Stem Cells
À utiliser avec (application)
Cell Culture, Differentiation
Quantité
1 Kit
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