For expansion and differentiation of human CD34+ hematopoietic progenitor cells to T cells
Type de cellule
Hematopoietic Stem and Progenitor Cells, T Cells
À utiliser avec (application)
Cell Culture; Differentiation; Expansion
Quantité
1 Kit
Marque
STEMCELL Technologies
Caractéristiques principales
Use this two-step protocol to reproducibly expand thousands of CD4+CD8+ double-positive (DP) T cells in cultures initiated with CD34+ human cord blood (CB) cells
In step one, CD34+ CB cells are cultured for 14 days in medium containing expansion supplement to promote their proliferation and differentiation into CD7+CD5+ pro-T cells
In step two, pro-T cells generated during those first 14 days are cultured for another 4 weeks in medium containing maturation supplement to promote their maturation into DP cells
This product is serum- and feeder-free, which helps eliminate variation introduced by serum and stromal cell lines
An optional protocol is available to further mature DP cells to CD8 SP T cells
This extended protocol uses complete T cell progenitor maturation medium made with reagents included in the StemSpan T Cell Generation Kit and which must be combined with ImmunoCult CD3/CD28/CD2 T Cell Activator (Catalog #10970 or #10971) and IL-15 (Catalog #78031)
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-031831
Réf. fabricant
17179961
Type de cellule
Hematopoietic Stem and Progenitor Cells, T Cells
À utiliser avec (application)
Cell Culture; Differentiation; Expansion
Quantité
1 Kit
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