ClonaCell™‐HY Medium D without HAT is a semi-solid methylcellulose-based medium that does not contain any selection agents such as HAT (hypoxanthine, aminopterin, and thymidine), enabling alternative hybridoma or myeloma selection methods to be used
This product can be used to select and clone myelomas or hybridomas in one step by adding an appropriate selection agent to the medium
Individual parental clones and their progeny remain localized together in the semi-solid matrix as they grow to form distinct colonies
This prevents the loss of rare clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of monoclonal colonies
The colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid medium for screening and expansion
