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STEMCELL Technologies ClonaCell™-CHO CD Medium | B-LABO France
STEMCELL Technologies ClonaCell™-CHO CD Medium
Réf. : BL-RE-031940Réf. fabricant : 17121036
1 096,50 € HT
Chemically defined, animal component-free, serum-free, protein-free, glutamine-free semi-solid methylcellulose-based medium for selection and cloning of CHO cells
Lignée cellulaire
CHO
À utiliser avec (application)
Cell Culture; Semi-Solid Cloning
Quantité
90 mL
Marque
STEMCELL Technologies
Caractéristiques principales
ClonaCell™-CHO CD Medium is a methylcellulose-based semi-solid medium recommended for selection and cloning of Chinese hamster ovary (CHO) cells
This medium is chemically defined, protein-free, and animal component-free
It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthetase (GS) selection systems
Benefits of semi-solid cloning: Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct monoclonal colonies
This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening
Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning
Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-031940
Réf. fabricant
17121036
Lignée cellulaire
CHO
À utiliser avec (application)
Cell Culture; Semi-Solid Cloning
Quantité
90 mL
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