HepatiCult™ Organoid Growth Medium (Mouse) is a serum-free, defined cell culture medium for establishment and maintenance of mouse hepatic progenitor organoids
These organoids, or 'mini-livers', provide an in vitro organotypic culture system for studying hepatic stem and progenitor cells
Organoids grown in HepatiCult™ feature an epithelium expressing genes marking hepatic stem and progenitor cells (PROM1, AXIN2, SOX9 and CD44), ducts (KRT19 and HNF1b) and hepatocytes (HNF4a, AFP)
Hepatic organoids can be passaged every 4 - 7 days, can be cryopreserved, and are primed for downstream differentiation
HepatiCult™ supports mouse hepatic organoid culture either embedded in Corning™ Matrigel™ domes or in a dilute Matrigel™ suspension
Organoid culture enables convenient in vitro characterization of the hepatic epithelium in a physiologically relevant system and reduces the need for animal use
