The LentiArray Human Druggable Genome Library is ideal for identifying potential therapeutic targets involved in the development and progression of disease. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC).
Points forts
Built to bring you success
LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific's proprietary CRISPR gRNA design algorithm. This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs. The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types.
Design and execute your experiments without limitations
LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells.
The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter. You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line. With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals.
Optimize your assay design and have full confidence in your results with LentiArray controls
High quality controls are the cornerstone of a successful screen. The LentiArray CRISPR libraries product line includes a suite of controls to help you quickly develop your assay and give you confidence in defining your hit criteria.
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-040930 |
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| Réf. fabricant | 18715515 |
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| Contenu et stockage | 1 ensemble de bibliothèques, 50 µl/cible.<br>Stocker entre -68 et -85°C. |
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| Conditions d’expédition | Glace carbonique |
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| À utiliser avec (application) | Modification génomique |
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| Type de produit | Bibliothèque Crispr |
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| Espèces | Humaine |
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| Quantité | 1 bibliothèque |
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| Type d’échantillon | ADN |
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| Format | Plaque 96 puits |
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| Gamme de produits | LentiArray™ |
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| RNAi Type | ARNg |
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