Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing
Type de cellule
Pluripotent Stem Cells
Quantité
20 nmol Kit
Marque
STEMCELL Technologies
Caractéristiques principales
ArciTect™ tracrRNA is a trans-activating CRISPR RNA (crRNA), one of two RNA components required to make a guide RNA (gRNA) for CRISPR-Cas9 genome editing
ArciTect™ tracrRNA contains a complementary sequence to the linker sequence in ArciTect™ crRNA, allowing fast and efficient gRNA duplex formation
It is recognized by all ArciTect™ Cas9 nucleases and is essential for ribonucleoprotein (RNP) complex formation
It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency
The ArciTect™ tracrRNA Kit includes tracrRNA as well as ArciTect™ Annealing Buffer (5X), which is required for forming a gRNA duplex between ArciTect™ tracrRNA and ArciTect™ crRNA
ArciTect™ crRNA is available by custom order for user-specified target sequences, in 2 nmol (Catalog #76010), 10 nmol (Catalog #76011), and 20 nmol (Catalog #76012) sizes
ArciTect™ Cas9 Nuclease is also available separately (Catalog #76001)
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-040975
Réf. fabricant
17109311
Type de cellule
Pluripotent Stem Cells
Quantité
20 nmol Kit
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