Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kits

Name: Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kits
Brand: Applied Biosystems
SKU: 18775455
Price: 3575.00 EUR
Availability: InStock

Réf. : BL-EQ-071005Réf. fabricant : 18775455 Assay Kit only

3 575,00 € HT

The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines.

Contenu et stockage: resDNASEQ HEK293 DNA Control
• HEK293 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C
• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use

resDNASEQ HEK293 DNA Real-Time PCR Reagents
• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• HEK293 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Méthode de détection: Primer-probe
Format: Kit
Méthode PCR: qPCR
Cellule hôte: HEK
Marque: Applied Biosystems

Points forts

Features of the resDNASEQ Quantitative HEK293 DNA Kit include

• Highly sensitive quantitation using proven TaqMan real-time qPCR technology

• Consistent performance across the expected range of DNA fragment sizes

Integrated workflow solution

The resDNASEQ Quantitative HEK293 DNA Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing. The kit is compatible with the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow. The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System. Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.

Easy to use

With the resDNASEQ Quantitative HEK293 DNA Kit, you perform qPCR analysis to compare the amount of HEK293 DNA in your test samples to a standard curve generated with known amounts of purified HEK293 DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to HEK293 genomic DNA standard, as well as step-by-step instructions.

Consistent performance

For accurate quantitation of residual HEK293 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.

*Due to the high degree of similarity between genomes, DNA from closely-related primate species, such as monkey (Vero), chimpanzee, and orangutan, are expected be detected by the resDNASEQ HEK293 Quantitation Kit.

Caractéristiques techniques

Marque	Applied Biosystems
Réf. B-LABO	BL-EQ-071005
Réf. fabricant	18775455
Contenu et stockage	<strong>resDNASEQ HEK293 DNA Control</strong><br /> • HEK293 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C<br /> • DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use<br /> <br /> <strong>resDNASEQ HEK293 DNA Real-Time PCR Reagents</strong><br /> • Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light<br /> • HEK293 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light<br /> • Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use<br /><br />
Méthode de détection	Primer-probe
Format	Kit
Méthode PCR	qPCR
Cellule hôte	HEK
Marqueur ou colorant	FAM
Gamme de produits	resDNASEQ™
Conditions d’expédition	Dry Ice
Nbre de réactions	100 Reactions
Comprend	Assay Kit only