The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells, an expression system commonly used for the production of recombinant proteins. Reliable and rapid, the resDNASEQ™ system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of Pichia pastoris cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types.
Points forts
Features of the resDNASEQ Quantitative Pichia pastoris DNA Kit include
• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
Easy to use
With the resDNASEQ™ Quantitative
Pichia pastoris
Specific to Pichia pastoris DNA, no cross-reactivity with unrelated DNA
The target of the assay is highly specific—it only detects a
Pichia pastoris
Consistent performance even with fragmented DNA
For accurate quantitation of residual
Pichia pastoris
Caractéristiques techniques
| Marque | Applied Biosystems |
| Réf. B-LABO | BL-EQ-071169 |
| Réf. fabricant | 30000666 |
| Contenu et stockage | <strong>resDNASEQ Pichia DNA Control</strong><br /> • Pichia DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C<br /> • DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use<br /> <br /> <strong>resDNASEQ Pichia DNA Real-Time PCR Reagents</strong><br /> • Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light<br /> • Pichia DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light<br /> • Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use<br /> <br /> <strong>PrepSEQ Nucleic Acid Extraction Kit</strong><br /> • Lysis Buffer (2 bottles, 50 mL/bottle)<br /> • Binding Solution/Isopropanol (1 empty bottle)<br /> • Wash Buffer Concentrate (2 bottles, 26 mL/b |
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| Méthode de détection | Primer-probe |
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| Format | Kit |
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| Méthode PCR | qPCR |
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| Cellule hôte | Pichia |
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| Marqueur ou colorant | FAM |
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| Gamme de produits | resDNASEQ™ |
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| Nbre de réactions | 100 Reactions |
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| Comprend | Assay Kit and Sample Preparation |
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