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Applied Biosystems™ resDNASEQ™ Quantitative Pichia pastoris DNA Kits

Réf. : BL-EQ-071169Réf. fabricant : 30000666 Assay Kit and Sample Preparation
3 965,00 € HT

The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells.

Contenu et stockage
resDNASEQ Pichia DNA Control
• Pichia DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C
• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use

resDNASEQ Pichia DNA Real-Time PCR Reagents
• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• Pichia DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use

PrepSEQ Nucleic Acid Extraction Kit
• Lysis Buffer (2 bottles, 50 mL/bottle)
• Binding Solution/Isopropanol (1 empty bottle)
• Wash Buffer Concentrate (2 bottles, 26 mL/b
Méthode de détection
Primer-probe
Format
Kit
Méthode PCR
qPCR
Cellule hôte
Pichia
Marque
Applied Biosystems

The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells, an expression system commonly used for the production of recombinant proteins. Reliable and rapid, the resDNASEQ™ system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of Pichia pastoris cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types.

Points forts

Features of the resDNASEQ Quantitative Pichia pastoris DNA Kit include

• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology

• Consistent performance across the expected range of DNA fragment sizes

Easy to use

With the resDNASEQ™ Quantitative

Pichia pastoris

Specific to Pichia pastoris DNA, no cross-reactivity with unrelated DNA

The target of the assay is highly specific—it only detects a

Pichia pastoris

Consistent performance even with fragmented DNA

For accurate quantitation of residual

Pichia pastoris

Caractéristiques techniques

MarqueApplied Biosystems
Réf. B-LABOBL-EQ-071169
Réf. fabricant30000666
Contenu et stockage<strong>resDNASEQ Pichia DNA Control</strong><br /> • Pichia DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C<br /> • DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use<br /> <br /> <strong>resDNASEQ Pichia DNA Real-Time PCR Reagents</strong><br /> • Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light<br /> • Pichia DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light<br /> • Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use<br /> <br /> <strong>PrepSEQ Nucleic Acid Extraction Kit</strong><br /> • Lysis Buffer (2 bottles, 50 mL/bottle)<br /> • Binding Solution/Isopropanol (1 empty bottle)<br /> • Wash Buffer Concentrate (2 bottles, 26 mL/b
Méthode de détectionPrimer-probe
FormatKit
Méthode PCRqPCR
Cellule hôtePichia
Marqueur ou colorantFAM
Gamme de produitsresDNASEQ™
Nbre de réactions100 Reactions
ComprendAssay Kit and Sample Preparation
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