Applied Biosystems™ resDNASEQ™ Quantitative NS0 DNA Kits

Name: Applied Biosystems™ resDNASEQ™ Quantitative NS0 DNA Kits
Brand: Applied Biosystems
SKU: 30000692
Price: 4235.00 EUR
Availability: InStock

Réf. : BL-EQ-071262Réf. fabricant : 30000692 Assay Kit and Sample Preparation

4 235,00 € HT

The resDNASEQ™ Quantitative NS0 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines.

Contenu et stockage: resDNASEQ NS0 DNA Control
• NS0 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C
• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use

resDNASEQ NS0 DNA Real-Time PCR Reagents
• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• NS0 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use

PrepSEQ Nucleic Acid Extraction Kit
• Lysis Buffer (2 bottles, 50 mL/bottle)
• Binding Solution/Isopropanol (1 empty bottle)
• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)
Méthode de détection: Primer-probe
Format: Kit
Méthode PCR: qPCR
Cellule hôte: NS0
Marque: Applied Biosystems

Points forts

Features of the resDNASEQ Quantitative NS0 DNA Kit include

• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology

• Consistent performance across the expected range of DNA fragment sizes

Easy to use, results in under 4 hours

The easy workflow of the resDNASEQ™ Quantitative NS0 DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform in under 4 hours. Start by extracting DNA from your in-process purification and drug substance samples. Then perform qPCR analysis to compare the amount of NS0 DNA in your test samples to a standard curve generated with known amounts of purified NS0 genomic DNA standard. The resDNASEQ™ system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan™ primer/probe mix to NS0 genomic DNA standard, as well as step-by-step instructions.

Specific to NS0 DNA, no cross-reactivity with unrelated DNA

The target of the assay is highly specific—it only detects a NS0-specific region of a multicopy genetic element. We selected this region using extensive bioinformatic analysis of multiple related and unrelated species.

Consistent performance even with fragmented DNA

For accurate quantitation of residual NS0 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ™ system you get consistent assay performance across a range of DNA fragment sizes.

Caractéristiques techniques

Marque	Applied Biosystems
Réf. B-LABO	BL-EQ-071262
Réf. fabricant	30000692
Contenu et stockage	<strong>resDNASEQ NS0 DNA Control</strong><br /> • NS0 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C<br /> • DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use<br /> <br /> <strong>resDNASEQ NS0 DNA Real-Time PCR Reagents</strong><br /> • Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light<br /> • NS0 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light<br /> • Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use<br /> <br /> <strong>PrepSEQ Nucleic Acid Extraction Kit</strong><br /> • Lysis Buffer (2 bottles, 50 mL/bottle)<br /> • Binding Solution/Isopropanol (1 empty bottle)<br /> • Wash Buffer Concentrate (2 bottles, 26 mL/bottle)<br />
Méthode de détection	Primer-probe
Format	Kit
Méthode PCR	qPCR
Cellule hôte	NS0
Marqueur ou colorant	FAM
Gamme de produits	resDNASEQ™
Nbre de réactions	100 Reactions
Comprend	Assay Kit and Sample Preparation