Detect both Legionella species and Legionella pneumophila from water samples with this rapid, sensitive and specific solution enabled by the qualyfast™ Legionella PCR Assay, Free DNA Inactivator, DNA Extraction and Quantification Standards kits.The assay independently detects both Legionella species and L. pneumophilia, includes inhibition control to minimize false-negative results, and offers the option for quantification by using calibrated standards. All consumables required for the workflow are included within the kits, with lyophilized and pre-dosed PCR reagents for ease-of-use.
Points forts
Minimizes false negatives
Free DNA Inactivator removes DNA from non-viable sources commonly found in environmental water samples.
DNA Extraction Kits remove environmental contaminants minimizing potential inhibition effects.
PCR Assays with simultaneous co-amplification of an Internal Application Control allowing determination of potential inhibition effects.
Increases accuracy
Quantification Standards enable PCR detection of <i>Legionella</i> species to be quantified within the 25-25,000 Genomic Unit range.
Increases throughput: Multiplexed PCR of the characteristic DNA sequences for species within the Legionella genus and the specific species L. pneumophilia.
Easy-to-use
PCR Assay kits contain strips with eight reaction tubes containing lyophilized PCR reagents, DNA free solution and pre-dosed positive-control tubes.
Quantification Standards are pre-aliquoted and lyophilized in individual tubes.
DNA Extraction Kits contain all the consumables required to extract DNA from environmental water samples.
Optimized workflow
The qualyfast® Legionella PCR Assay contains:
The qualyfast® DNA Extraction Kit I is designed for use with clear water samples, as acquired from water distribution systems, drinking water and other sources that contain minimal suspended particles. The kit contains:
Complete end-to-end workflow covering sample preparation using DNA Extraction Kits and Free DNA Inactivator, detection using multiplex Legionella PCR Assay and evaluation of results using Quantification Standards
ISO compliant: workflow is compliant with the protocol, experimental design and calculation methods described by ISO/TS 12869:2012
Pre-dosed and lyophilized PCR reagents in strips of 8 reaction tubes
A strip of 8 reaction tubes containing positive controls
DNA-free solution
Single-use, sterile membrane filters
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-EQ-071321
Réf. fabricant
17879256
Méthode de détection
qPCR
Forme
Lyophilized
Format
Tube
GC-Rich PCR Performance
Low
Méthode PCR
qPCR
Vitesse de réaction
Fast
Nbre de tests
8 Tests
Type de produit
Legionella PCR and DNA Extraction Kit
Suffisant pour
8 Reactions
À utiliser avec (application)
Pathogen Detection, DNA Extraction
Nbre de réactions
8 Reactions
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