Propidium Iodide (PI) is a red-fluorescent cell viability dye which is excluded from live cells with intact membranes, but penetrates dead or damaged cells and binds to DNA and RNA by intercalating between the bases
It is widely used as a counterstain to differentiate and exclude non-viable cells in flow cytometric analyses, and can be excited using blue (488 nm), green (532 nm), or yellow-green (561 nm) laser lines, with detection in the FL2 or FL3 channels
PI is used in DNA fluorescence imaging applications to discriminate early and late stages of apoptosis, to study cell‐mediated cytotoxicity, and for chromosome analysis
It is also commonly used in quantitative DNA assays
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-086852
Réf. fabricant
17180352
CAS
25535-16-4
Quantité
10 mg
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