"/> STEMCELL Technologies DAPI (Hydrochloride) | B-LABO France

STEMCELL Technologies DAPI (Hydrochloride)

Réf. : BL-RE-086853Réf. fabricant : 17100362 10 mg
222,75 € HT

DNA-labeling dye

CAS
28718-90-3
Quantité
10 mg
Marque
STEMCELL Technologies

Caractéristiques principales

  • DAPI (4',6-diamidino-2-phenylindole dihydrochloride) is a blue-fluorescent dye that binds to AT-rich regions of double-stranded DNA
  • Binding is accompanied by an ∼20-fold enhancement in fluorescence, which is directly proportional to the amount of DNA present and has an emission maximum at ∼454 nm
  • The complex is stable for several hours at room temperature and over the pH range 4 - 11
  • DAPI can also bind to RNA, evidently through AU-selective intercalation, though the DAPI/RNA complex emits at a longer wavelength (500 nm) and with only an ∼20% increase in quantum yield
  • DAPI has been widely used as a counterstain to detect nuclei in multicolor fluorescence applications, where its blue fluorescence vividly contrasts with red, yellow, or green fluorescent dyes used to stain other structures

Caractéristiques techniques

MarqueSTEMCELL Technologies
Réf. B-LABOBL-RE-086853
Réf. fabricant17100362
CAS28718-90-3
Quantité10 mg
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