DAPI (4',6-diamidino-2-phenylindole dihydrochloride) is a blue-fluorescent dye that binds to AT-rich regions of double-stranded DNA
Binding is accompanied by an ∼20-fold enhancement in fluorescence, which is directly proportional to the amount of DNA present and has an emission maximum at ∼454 nm
The complex is stable for several hours at room temperature and over the pH range 4 - 11
DAPI can also bind to RNA, evidently through AU-selective intercalation, though the DAPI/RNA complex emits at a longer wavelength (500 nm) and with only an ∼20% increase in quantum yield
DAPI has been widely used as a counterstain to detect nuclei in multicolor fluorescence applications, where its blue fluorescence vividly contrasts with red, yellow, or green fluorescent dyes used to stain other structures
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-086853
Réf. fabricant
17100362
CAS
28718-90-3
Quantité
10 mg
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