Calcofluor White Stain Kit is a fluorescent stain used for the rapid detection of fungi, Microsporidium, Acanthamoeba, and Pneumocystis.
Use Thermo Scientific™ Remel™ Calcofluor White Stain Kit as a rapid, non-specific fluorochrome stain for the initial microscopic detection of fungal elements, yeasts, Acanthamoeba cysts, microsporidia, and Pneumocystis carinii in clinical specimens. Hageage and Harrington described the use of calcofluor white (CFW) to demonstrate hyphae and yeasts in clinical specimens1. More recently calcofluor white has been used to detect fungi in direct preparations and in deparaffinized tissue sections2,3.
Calcofluor White Stain Kit is a fluorescent stain for rapid detection of yeasts, fungi and parasitic organisms. Calcofluor White is a non-specific fluorochrom that binds to cellucose and chitin in cell walls. Upon excitation with long wave ultraviolet light, this compound delineates the cell walls of cellulose-containing organisms2. Addition of clearing agent such as potassium hydroxide to the specimen helps in dissolving tissue cells prior to staining with calcofluor white. Evans blue dye is incorporated in the stain to minimize background material.
Kit includes the reagents such as Potassium Hydroxide (Reagent A) along with Calcofluor White and Evans Blue Dye (Reagent B).
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| Marque | Thermo Scientific |
|---|---|
| Réf. B-LABO | BL-RE-086890 |
| Réf. fabricant | 16295120 |
| Volume (métrique) | 50 mL/Bottle |
| Type | Calcofluor White Stain |
| Quantité | 2/kit |
| Comprend | 1 bottle Calcofluor White; 1 bottle KOH |
