Synthétise 10 à 50 fois la quantité d’ARN produite par les réactions de transcription classiques.
Contenu et stockage
SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at -20°C. Nuclease-free Water may be stored at any temperature.
À utiliser avec (application)
Gene Expression Analysis and Genotyping, in vitro Transcription
Comprend
SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, and Nuclease-free Water
Nbre de réactions
40
Type de produit
Kit with manual
Marque
Invitrogen
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10 to 50 times the amount of RNA produced by conventional transcription reactions. Each Ambion kit includes sufficient reagents for 40 reactions.
Caractéristiques principales
Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
Amplifies aRNA for gene array analysis and other applications
Efficiently incorporates many modified nucleotides
A typical 20μL MEGAscript reaction with 1μg of the pTRI-Xef-1α control template will yield over 100μg of transcript
Versatility of the MEGAscript kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase
Accessory Products:
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use MEGAclear™ Kit (Mfr. No. AM1908) and the MEGAclear-96 Kit (Mfr. No. AM1909).
Caractéristiques techniques
Marque
Invitrogen
Réf. B-LABO
BL-RE-087018
Réf. fabricant
10418644
Contenu et stockage
SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at -20°C. Nuclease-free Water may be stored at any temperature.
À utiliser avec (application)
Gene Expression Analysis and Genotyping, in vitro Transcription
Comprend
SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, and Nuclease-free Water
Nbre de réactions
40
Type de produit
Kit with manual
Une question, une configuration particulière ? Contactez-nous pour un devis.
