Tarif personnalisé selon la configuration et la quantité.
The Applied Biosystems™ HIV-1 Genotyping Kit with Integrase is designed to genotype the protease, reverse transcriptase (RT), and integrase (IN) regions of the HIV-1 pol gene. The genotyping result encompasses codons 6-99 in the protease region and codons 1-251 in the RT region of the gene.
Contenu et stockage
• RNA Positive Control, 2 x 100 μL • HIV RNA Negative Control, 2 x 100 μL • RT-PCR Master Mix, PR/RT, 2 x 1.055 mL • RT-PCR Master Mix, IN, 2 x 1.055 mL • Nested-PCR Master Mix, PR/RT, 2 x 1.285 mL • Nested-PCR Master Mix, IN, 2 x 1.285 mL • SuperScript III One-Step RT-PCR with Platinum Taq High Fidelity Enzyme, 2 x 54 μL • AmpliTaq Gold LD DNA Polymerase, 1 x 54 μL • Sequencing vector, 2 x 80 μL • HIV Sequencing Mix R12, 2 x 435 μL • HIV Sequencing Mix R11, 2 x 435 μL • HIV Sequencing Mix F12, 2 x 435 μL • HIV Sequencing Mix F11, 2 x 435 μL • HIV Sequencing Mix R3, 2 x 435 μL • HIV Sequencing Mix R2, 2 x 435 μL • HIV Sequencing Mix R1, 2 x 435 μL • HIV Sequencing Mix F3, 2 x 435 μL • HIV Sequencing Mix F2, 2 x 435 μL • HIV Sequencing Mix F1, 2 x 435 μL
Designed to genotype the protease, reverse transcriptase (RT) and integrase (IN) regions of the HIV-1 pol gene
The genotyping result encompasses codons 6-99 in the protease region and codons 1-251 in the RT region of the gene
HIV-1 genomic mutations in these regions confer resistance to anti-retroviral drugs that act through the inhibition of the protease and reverse transcriptase
The kit enables reliable genotyping of the genetically diverse HIV-1 virus to investigate Drug Resistance Mutations (DRMs) to protease inhibitors (PI) , nucleoside reverse-transcriptase inhibitors (NRTI), non-nucleoside reverse-transcriptase inhibitors (NNRTI), and integrase inhibitors (INSTI) from HIV-1 RNA extracted from EDTA plasma and dried blood spot samples
This product employs assays for HIV-1 genotyping licensed from the U.S. Centers for Disease Control and Prevention
Kit contains ready-to-use master mix, enzyme, and positive and negative controls to cover the three phases of the workflow: RT-PCR, nested PCR, and bidirectional cycle sequencing steps to process 48 test samples
Starting with viral RNA extracted from an EDTA plasma or dried blood spot sample, RT-PCR is conducted to generate PR/RT and IN PCR products as DNA template
After confirming the band size of the PCR amplicons, the Cycle Sequencing module is used to sequence the DNA templates utilizing Sanger sequencing technology
The sequencing reactions are first purified, then sequenced using POP-7 on a designated capillary electrophoresis system
The sequencing traces are reviewed for quality, after which the user exports the AB1 file for use in downstream analysis and reporting software a mutation profile through a curated database
Caractéristiques techniques
Marque
Applied Biosystems
Réf. B-LABO
BL-RE-087233
Réf. fabricant
17767517
Contenu et stockage
• RNA Positive Control, 2 x 100 μL<br />• HIV RNA Negative Control, 2 x 100 μL<br />• RT-PCR Master Mix, PR/RT, 2 x 1.055 mL<br />• RT-PCR Master Mix, IN, 2 x 1.055 mL<br />• Nested-PCR Master Mix, PR/RT, 2 x 1.285 mL<br />• Nested-PCR Master Mix, IN, 2 x 1.285 mL<br />• SuperScript III One-Step RT-PCR with Platinum Taq High Fidelity Enzyme, 2 x 54 μL<br />• AmpliTaq Gold LD DNA Polymerase, 1 x 54 μL<br />• Sequencing vector, 2 x 80 μL<br />• HIV Sequencing Mix R12, 2 x 435 μL<br />• HIV Sequencing Mix R11, 2 x 435 μL<br />• HIV Sequencing Mix F12, 2 x 435 μL<br />• HIV Sequencing Mix F11, 2 x 435 μL<br />• HIV Sequencing Mix R3, 2 x 435 μL<br />• HIV Sequencing Mix R2, 2 x 435 μL<br />• HIV Sequencing Mix R1, 2 x 435 μL<br />• HIV Sequencing Mix F3, 2 x 435 μL<br />• HIV Sequencing Mix F2, 2 x 435 μL<br />• HIV Sequencing Mix F1, 2 x 435 μL<b
Format
Kit
Isolation Technology
Magnetic Bead
Type d’échantillon
RNA from EDTA Plasma and DBS
Sequencing Type
DNA and RNA Sequencing
À utiliser avec (équipement)
Veriti™ 96‐well Thermal Cycler with 0.2‐mL sample wells, GeneAmp™ PCR System 9700 96-well, ProFlex™ 96‐well PCR System with 0.2 mL sample wells, 3130 series Sequencers, 3500/3500xL Genetic Analyzer series, SeqStudio Flex Genetic Analyzers (8 or 24 caps, up to 4 plates)
