The MicroSEQ ID Purification Combo Kit v2.0 includes reagents for post-PCR and post-sequencing clean-up in the MicroSEQ ID microbial identification workflow.
Contenu et stockage
• ExoSAP-IT Express (100 reactions), store at -5°C to -30°C • MicroSEQ ID Sequencing Clean-up Cartridges (36 cartridges), store at 2°C to 8°C
Format
Cartridge
À utiliser avec (application)
Sequencing
Compatibilité à haut débit
Manual Protocols
Nbre de réactions
100 Reactions
Marque
Applied Biosystems
ExoSAP-IT Express Reagent removes unincorporated dNTPs and primers from the post-amplification PCR mix in 5 minutes compared to 30 minutes with the original ExoSAP-IT Reagent while producing equivalent results. The MicroSEQ ID Sequencing Clean-up Cartridges provide a convenient and simple way to remove dye terminators, dNTPs, salts, and residual primers from cycle sequencing reactions. The end products can be used directly for sequencing on any Applied Biosystems genetic analyzer.
Comparability studies demonstrated equivalent results between the original ExoSAP-IT Reagent and the improved ExoSAP-IT Express Reagent coupled with the MicroSEQ ID Sequencing Clean-Up kit in the MicroSEQ ID workflow (see figure 1). Both ExoSAP-IT and ExoSAP-IT Express enzyme-treated PCR samples produced very similar sequencing results and the correct identification result.
ExoSAP-IT Express Reagent can be used downstream of the following PCR amplification kits:
MicroSEQ ID Sequencing Clean-up Cartridges can be used downstream of the following sequencing kits:
Caractéristiques principales
MicroSEQ 500 16S rDNA PCR Kit
MicroSEQ Full Gene 16S rDNA PCR Kit
MicroSEQD2 rDNA Fungal PCR Kit
MicroSEQ 500 16S rDNA Sequencing Kit
MicroSEQ Full Gene 16S rDNA Sequencing Kit
MicroSEQ D2 rDNA Fungal Sequencing Kit
Caractéristiques techniques
Marque
Applied Biosystems
Réf. B-LABO
BL-RE-088889
Réf. fabricant
18725525
Contenu et stockage
• ExoSAP-IT Express (100 reactions), store at -5°C to -30°C<br>• MicroSEQ ID Sequencing Clean-up Cartridges (36 cartridges), store at 2°C to 8°C
Format
Cartridge
À utiliser avec (application)
Sequencing
Compatibilité à haut débit
Manual Protocols
Nbre de réactions
100 Reactions
Gamme de produits
MicroSEQ™
Quantité
1 kit
Conditions d’expédition
Approved for shipment on Wet or Dry Ice
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