Designed for fast and efficient cleanup of pre-purified DNA samples. Removes a wide range of inhibitory compounds, such as those found in environmental samples (humic acid, heavy metals, etc.), as well as organic compounds that may co-purify with DNA from other extraction procedures.
Contenu et stockage
Store at Room Temperature, Dry Environment
Isolation Technology
Spin Column
Type d’échantillon
25 to 200 µL DNA preparations > 200 bp
Comprend
Buffer P1 50 mL, Buffer P1HA 50mL, Buffer P2 40 mL, Buffer P3 40 mL, Buffer P4 6 mL, 50x Column P, 100 x 2.0 mL collection tubes, 50 x 1.5 mL collection tubes
Quantité
50 Preps
Marque
MP Biomedicals
Caractéristiques principales
The SPINeasy DNA Purification Kit is designed for the removal of contaminants that may co-purify with DNA and inhibit downstream enzymatic, PCR applications.
Inhibitory compounds such as those found in environmental samples (humic acid, heavy metals, etc.), as well as organic compounds that may co-purify with DNA from other extraction procedures, are efficiently and quickly removed using this kit.
The kit offers two choices of binding buffers. Buffer P1 is recommended for purification of samples with slight contamination of humic acids (light brownish color) or other PCR inhibitors. Buffer P1HA contains a novel and proprietary humic acid removal technology, which is recommended for processing samples contaminated with a considerable amount of humic substances (strong brownish color).
The kit has been validated with genomic DNA isolated from various problematic soil samples, as well as with DNA samples spiked with humic acid molecules. The data shows that the kit can clean up to 50 µg DNA with a size ranging from 200 bp to high molecular weight, with expected recovery of > 80%.
Cleaned-up DNA samples can be readily used in downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome).
Caractéristiques techniques
Marque
MP Biomedicals
Réf. B-LABO
BL-RE-089166
Réf. fabricant
17864126
Contenu et stockage
Store at Room Temperature, Dry Environment
Isolation Technology
Spin Column
Type d’échantillon
25 to 200 µL DNA preparations > 200 bp
Comprend
Buffer P1 50 mL, Buffer P1HA 50mL, Buffer P2 40 mL, Buffer P3 40 mL, Buffer P4 6 mL, 50x Column P, 100 x 2.0 mL collection tubes, 50 x 1.5 mL collection tubes
Quantité
50 Preps
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