Applications: Retroviral Production
The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a sell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.
| Marque | Novus Biologicals |
|---|---|
| Réf. B-LABO | BL-RE-090263 |
| Réf. fabricant | 18736093 |
| Tampon de stockage | 10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA) |
| Contenu et stockage | Store at -20°C. Avoid freeze-thaw cycles. |
| Quantité | 10 μg |
| Vecteur | pCL-Eco Retrovirus Packaging Vector |
